CD4 Antibody
-
Catalog number6937-50
-
PricePlease ask
-
Size50 µl
-
-
Antibody TargetCD4
-
Target Alternative NameT-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4
-
Antibody TypePolyclonal
-
Species ReactivitiesHuman, Mouse, Rat, Dog, Monkey
-
Immunogen SequenceThis CD4 antibody is generated from KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD4. The exact sequence is proprietary.
-
AccessionP01730
-
Gene Id920
-
AppearanceColorless liquid
-
FormLiquid
-
Concentration1 mg/ml
-
Formulation1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
-
PurificationImmunogen Affinity chromatography
-
ApplicationWB
-
Positive ControlWB: SP20, PC12, H9C2 whole cell lysate
-
Application And UsagesWB: 1:500 - 1:1000
-
DescriptionCD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with have either CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedVerified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Dot Blot VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Additional descriptionCD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
-
French translationanticorps
-
Gene target
-
Gene symbolCD4
-
Short nameCD4 Antibody
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameCD4 molecule (antibody to-)
-
Alternative techniqueantibodies
-
Alternative to gene targetCD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
-
Gene info
-
Identity
-
Gene
-
Long gene nameCD4 molecule
-
Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
-
GenBank acession
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
RefSeq identity
-
Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data