Polyclonal (rabbit origin)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. H3 is a replication-independent member of the histone family.
Antigen affinity purified
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
A portion of amino acids 1-30 from the human protein was used as the immunogen for this Histone H3.1 antibody.
The stated application concentrations are suggested starting amounts. Titration of the Histone H3.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Rabbit (Oryctolagus cuniculus)
Western blot: 1:1000
In 1X PBS, pH 7.4, with 0.09% sodium azide
This Histone H3.1 antibodyis to be used only for research purposes and not for diagnostics..
Store at 4oC for up to one month. For long term, aliquot the Histone H3.1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.