Recombinant Human Glial Cell Line-Derived Neurotrophic Factor/GDNF Protein 50µg
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Catalog numberPKSH032488-50µg
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PricePlease ask
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Size50µg
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SynonymGlial Cell Line-Derived Neurotrophic Factor; hGDNF; Astrocyte-Derived Trophic Factor; ATF; GDNF
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ActivityMeasured by its ability to bind Human GFRA1 in functional ELISA.Activated
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SequenceSer78-Ile211
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Fusion tagNA
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AccessionP39905
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Expressed HostE.coli
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ShippingThe product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
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Purity>95% as determined by reducing SDS-PAGE.
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Endotoxin<1.0 EU per µg as determined by LAL test.
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Stability and StorageLyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
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Mol Mass15.1 kDa
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AP Mol Mass17 kDa
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FormulationLyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.25.
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ReconstitutionPlease refer to it for detailed information.
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BackgroundGlial Cell Line-Derived Neurotrophic Factor (GDNF) is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-β superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.
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DescriptionFor cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolGDNF, GDNF-AS1
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Short nameRecombinant Glial Line-Derived Neurotrophic Factor/GDNF Protein 50µg
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens Glial cellular Line-Derived Neurotrophic Factor/glial cell derived neurotrophic factor Protein 50µg
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Alternative techniquerec
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Alternative to gene targetglial cell derived neurotrophic factor, ATF1 and ATF2 and HFB1-GDNF and HSCR3, GDNF and IDBG-17169 and ENSG00000168621 and 2668, protein homodimerization activity, Extracellular, Gdnf and IDBG-128378 and ENSMUSG00000022144 and 14573, BT.58072 and IDBG-630047 and ENSBTAG00000005176 and 386587
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameglial cell derived neurotrophic factor
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Synonyms
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Synonyms name
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Locus
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Discovery year1995-05-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GDNF family ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameGDNF antisense RNA 1
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Synonyms gene name
- GDNF antisense RNA 1 (non-protein coding)
- GDNF antisense RNA 1
- GDNF antisense RNA 1 (head to head)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2011-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data