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Long name
Acetyl-Histone H4(K5) Antibody, ALEXA FLUOR 647 Conjugated
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Also known as
Anti-Acetyl-Histone H4K5 PAb ALEXA FLUOR 647
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 647
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Acetyl-Histone H4K5
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Specificity
This is a highly specific antibody against Acetyl-Histone H4K5.
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Modification
Acetylation
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Modification Site
K5
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
KLH conjugated synthetic acetylpeptide derived from human Histone H4 around acetylation site of K5
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Gene ID Number
121504
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
650nm/665nm
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Synonyms
Histone H4 Acetyl K5; Histone H4 Acetyl Lys5; Acetyl-Histone H4 Lys5; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN.
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Description
N terminal acetylation or CH3CO as epigenetic regulation of Anti-Acetyl-Histone H4K5 by NATs.
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-Acetyl-Histone H4K5 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR 647
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French translation
anticorps