Goat, Goat anti-biotin, ALP conjugated

• Catalog number
  AS10 1452
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Liquid
• Immunogen
  Biotin
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified IgG
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
• Verified applications
  Contact Gentaur support center
• Connected products
  other secondary antibodies against biotin
• Recommended dilutions for use
  This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500 - 1:2000 for Immunohistochemistry 1:50 000 - 1:5 000 for ELISA/Western blot
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  Refer to PubMed
• Scientific context
  Goat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
• Notes
  Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Latin name
  Capra aegagrus hircus

• Gene target
  ALP   conjugated  
• Gene symbol
  PDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
• Short name
  Goat, Goat anti- , ALP conjugated
• Technique
  anti-, anti, Goat, antibody to, antibodies, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  Biotin
• Species
  Goats
• Alternative name
  caprine, caprine Antibody tobiotinilated, ALP coupled
• Alternative technique
  antibodies, caprine

Gene info

• Identity
  HGNC:20767
• Gene
  PDLIM3
• Long gene name
  PDZ and LIM domain 3
• Synonyms
  • ALP
• GenBank acession
  • AF002280
• Locus
  4q35.1
• Discovery year
  2004-02-06
• Entrez gene record
  27295
• Pubmed identfication
  • 10063829
  • 8828038
• RefSeq identity
  • NM_014476
• Classification
  • PDZ domain containing
  • LIM domain containing
• VEGA ID
  OTTHUMG00000160412
• Locus Specific Databases
  • LRG_752

Gene info

• Identity
  HGNC:16448
• Gene
  ASRGL1
• Long gene name
  asparaginase and isoaspartyl peptidase 1
• Synonyms gene name
  • asparaginase like 1
• Synonyms
  • FLJ22316
  • ALP1
  • ALP
• Synonyms name
  • asparaginase-like 1 protein
• Locus
  11q12.3
• Discovery year
  2001-08-29
• Entrez gene record
  80150
• Pubmed identfication
  • 11984834
  • 28671681
  • 27106100
• RefSeq identity
  • NM_001083926
• VEGA ID
  OTTHUMG00000167513

Gene info

• Identity
  HGNC:29063
• Gene
  ATRNL1
• Long gene name
  attractin like 1
• Synonyms gene name
  • attractin-like 1
• Synonyms
  • KIAA0534
  • FLJ45344
  • ALP
• GenBank acession
  • AB011106
• Locus
  10q25.3
• Discovery year
  2004-03-04
• Entrez gene record
  26033
• Pubmed identfication
  • 9628581
• RefSeq identity
  • NM_207303
• Classification
  • C-type lectin domain containing
• VEGA ID
  OTTHUMG00000019096

Gene info

• Identity
  HGNC:11092
• Gene
  SLPI
• Long gene name
  secretory leukocyte peptidase inhibitor
• Synonyms gene name
  • secretory leukocyte protease inhibitor (antileukoproteinase)
• Synonyms
  • HUSI-I
  • ALK1
  • ALP
  • BLPI
  • HUSI
  • WAP4
  • WFDC4
• Synonyms name
  • antileukoproteinase
• GenBank acession
  • X04502
• Locus
  20q13.12
• Discovery year
  1998-04-24
• Entrez gene record
  6590
• Pubmed identfication
  • 3640338
  • 12206714
• Classification
  • WAP four-disulfide core domain containing
• VEGA ID
  OTTHUMG00000033075

Gene info

• Identity
  HGNC:10626
• Gene
  CCL27
• Long gene name
  C-C motif chemokine ligand 27
• Synonyms gene
  • SCYA27
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 27
  • chemokine (C-C motif) ligand 27
• Synonyms
  • ALP
  • ILC
  • CTACK
  • skinkine
  • ESkine
  • PESKY
  • CTAK
• Synonyms name
  • CC chemokine ILC
  • IL-11 Ralpha-locus chemokine
  • cutaneous T-cell attracting chemokine
• GenBank acession
  • AJ243542
• Locus
  9p13.3
• Discovery year
  1999-07-30
• Entrez gene record
  10850
• Pubmed identfication
  • 10556532
  • 10588729
• RefSeq identity
  • NM_006664
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000019834

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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