Reduced Glutathione (GSH) Assay Kit (Colorimetric)
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Catalog number
K464-100
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Price
Please ask
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Size
100 assays
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Description
It specifically detects reduced glutathione. Oxidized GSSG is not detected.
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Summary
• Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.
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Detection Method
Detection method- Absorbance (450 nm)
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Species Reactivity
Mammalian
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Applications
The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.
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Sample Type
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes only ~1-2 hours • Fast and convenient • Specific: does not detect oxidized GSSG • Sensitive: detects as low as 50 pmol GSH
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glutathione (GSH), a thiol-containing tripeptide (γ-glutamyl-cysteinyl-glycine), is a key antioxidant in many species. It has been highly implicated in the detoxification/elimination of xenobiotics (naturally occurring harmful compounds such as free radicals, hydroperoxides etc.) and in the maintenance of the oxidation state of protein sulfhydryl groups. In addition, GSH plays a pivotal role in the pathogenesis of numerous human diseases including cancer and cardio-vascular diseases. Glutathione is present in cells in both reduced (GSH) and oxidized (GSSG) forms- GSH being, the predominant species under normal physiological conditions inside cells. Thus, pathologic conditions causing oxidative stress would result in increased levels of GSSG. Therefore, the measurement of intracellular GSH appears to be a sensitive indicator of the overall cell health, and its ability of resisting toxic challenges. BioVision’s Reduced Glutathione Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at 450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample. Our kit is the most specific and sensitive quantitative assay in the market because GSSG does not interfere with the assay. The kit includes 5-Sulfosalicylic acid (SSA) in order to protect samples’ GSH endogenous content. (SSA acts as a deproteinizing and antioxidant agent. The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbol
GSX1
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Short name
Reduced Glutathione (GSH) Assay Kit (Colorimetric)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Reduced GSH (GSH) test reagent (colorimetry)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
GS homeobox 1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-02-18
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
Product images
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