WISP-1, Human Recombinant

• Catalog number
  P1281-5
• Price
  Please ask
• Size
  5 ug

• Synonyms
  WNT-1 inducible signaling pathway protein-1, CCN4, Wnt-1-induced secreted protein, WISP-1
• Alternative_names
  WNT-1 inducible signaling pathway protein-1, CCN4, Wnt-1-induced secreted protein, WISP-1
• Description
  Belongs to CCN family of cysteine rich regulatory proteins.
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  ≥ 98%
• Assay
  SDS-PAGE
• Purity
  ≥ 98%
• Biological activity
  Determined by the dose-dependent proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 µg/ml.
• Molecular Weight
  38.1 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 10 mM Acetic Acid.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  WISP-1 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is expressed in the heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. WISP-1 is a β-catenin regulated protein that can contribute to tumorigenesis and has also been shown to play a role in bone development and fracture repair. Human WISP-1 is a 38.1 kDa protein containing 346 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor-C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain.
• Amino acid sequence
  TALSPAPTT MDFTPAPLE DTSSRPQFC KWPCECPPS PPRCPLGVS LITDGCECC KMCAQQLGD NCTEAAICD PHRGLYCDY SGDRPRYAI GVCAQVVGV GCVLDGVRY NNGQSFQPN CKYNCTCID GAVGCTPLC LRVRPPRLW CPHPRRVSI PGHCCEQWV CEDDAKRPR KTAPRDTGA FDAVGEVEA WHRNCIAYT SPWSPCSTS CGLGVSTRI SNVNAQCWP EQESRLCNL RPCDVDIHT LIKAGKKCL AVYQPEASM NFTLAGCIS TRSYQPKYC GVCMDNRCC IPYKSKTID VSFQCPDGL GFSRQVLWI NACFCNLSC RNPNDIFAD LESYPDFSE IAN
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  WISP-1  
• Gene symbol
  CCN4, CCN6, CCN5, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1
• Short name
  WISP-1, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  WISP-1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:12769
• Gene
  CCN4
• Long gene name
  cellular communication network factor 4
• Synonyms gene
  • WISP1-OT1
  • WISP1-UT1
  • WISP1
• Synonyms gene name
  • WISP1 overlapping transcript 1
  • WISP1 3'UTR-associated RNA 1
  • WISP1 overlapping transcript 1 (non-protein coding)
  • WNT1 inducible signaling pathway protein 1
• Synonyms
  • WISP-1
• GenBank acession
  • AF100779
• Locus
  8q24.22
• Discovery year
  1999-01-22
• Entrez gene record
  8840
• Pubmed identfication
  • 9843955
• RefSeq identity
  • NM_003882
• Classification
  • Cellular communication network factors
• VEGA ID
  OTTHUMG00000164440

Gene info

• Identity
  HGNC:12771
• Gene
  CCN6
• Long gene name
  cellular communication network factor 6
• Synonyms gene
  • WISP3
• Synonyms gene name
  • WNT1 inducible signaling pathway protein 3
• Synonyms
  • WISP-3
• GenBank acession
  • AF100781
• Locus
  6q21
• Discovery year
  1999-01-22
• Entrez gene record
  8838
• Pubmed identfication
  • 9843955
• RefSeq identity
  • NM_003880
• Classification
  • Cellular communication network factors
• VEGA ID
  OTTHUMG00000185101

Gene info

• Identity
  HGNC:12770
• Gene
  CCN5
• Long gene name
  cellular communication network factor 5
• Synonyms gene
  • WISP2
• Synonyms gene name
  • WNT1 inducible signaling pathway protein 2
• Synonyms
  • CT58
  • CTGF-L
  • WISP-2
• GenBank acession
  • AF100780
• Locus
  20q13.12
• Discovery year
  1999-01-22
• Entrez gene record
  8839
• Pubmed identfication
  • 9843955
• RefSeq identity
  • NM_003881
• Classification
  • Cellular communication network factors
• VEGA ID
  OTTHUMG00000033071

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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