Hamster Anti-Mouse KLRG1/MAFA-FITC
-
Catalog number
GEN673576
-
Price
Please ask
-
Size
500ug
-
-
Also known as
KLRG1
-
Other names
N/A
-
Category
Secondary Antibodies
-
Subcategory
Mnoclonal antibodies
-
Gene name
N/A
-
Gene name synonims
N/A
-
Other gene names
N/A
-
Clonality
Monoclonal
-
Immunoglobulin isotype
Syrian Hamster IgG
-
Clone
2F1
-
Host organism
Syrian Hamster
-
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
-
Specificity and cross reactivity
KLRG1 (Mr 30-38 kDa) The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1-Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains.1, 2 These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1.3, 4 The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).1, 5 Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.1; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
-
Purification method
N/A
-
Form Appearance
Fluorescein (FITC) Conjugate
-
Concentration
N/A
-
Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
-
Tested applications
Flow Cytometry; Immunoprecipitation
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
-
Conjugation
Anti-FITC Antibody
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Gene symbol
KLRG1, MAFA, MAFA-AS1
-
Short name
Anti-Mouse KLRG1/MAFA-FITC
-
Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
-
Host
Hamster
-
Label
FITC
-
Species
Mouse, Hamsters, Mouses
-
Alternative name
Anti-Hamster Anti-Mouse KLRG1/MAFA-FITC
-
Alternative technique
murine, antibodies, fluorescine
-
Alternative to gene target
killer cell lectin-like receptor subfamily G, member 1, 2F1 and CLEC15A and MAFA and MAFA-2F1 and MAFA-L and MAFA-LIKE, KLRG1 and IDBG-17564 and ENSG00000139187 and 10219, carbohydrate binding, Plasma membranes, Klrg1 and IDBG-185261 and ENSMUSG00000030114 and 50928, KLRG1 and IDBG-639693 and ENSBTAG00000013640 and 100295672
-
Gene info
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
MAFA antisense RNA 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year
2014-08-07
-
Pubmed identfication
-
Classification
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
