FGF-2/FGF-basic, human recombinant
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Catalog number4037-1000
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PricePlease ask
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Size1 mg
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SynonymsProstatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast Growth Factor
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Alternative_namesFGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
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DescriptionRecombinant FGF2 from E.Coli, animal origin free, ≥95% pure, biologically active, cell culture tested. A growth factor involved in angiogenesis, wound healing and embryonic development.
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Purity≥95%
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Biological activityThe ED₅₀ of human FGF-2 is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 3 ng/ml.
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Molecular WeightHuman FGF-2 is a 17.2 kDa recombinant protein
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceRecombinant FGF-2 is available as a lyophilized powder
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Physical form descriptionFGF-2 protein is lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 + 150 mM NaCl
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Reconstitution InstructionsReconstitute human FGF-2 in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
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Background InformationFGF-2 (FGF2, FGF 2, FGF-basic, basic FGF, bFGF) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-2 protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different FGF-2 isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional descriptionBasic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolFGF18, FGF17, NUDT6, FGF2, FGF4, FGF1, FGF13, MIR7-2, MIR329-2, MIR512-2
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Short nameFGF-2/FGF- , recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameFGF-2/FGF-basic, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor 18
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibroblast growth factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor 17
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibroblast growth factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenudix hydrolase 6
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Synonyms gene name
- nudix (nucleoside diphosphate linked moiety X)-type motif 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nudix hydrolase family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor 2
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Synonyms gene
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Synonyms gene name
- fibroblast growth factor 2 (basic)
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibroblast growth factor family
- Receptor ligands
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor 4
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Synonyms gene
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Synonyms gene name
- heparin secretory transforming protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibroblast growth factor family
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor 1
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Synonyms gene
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Synonyms gene name
- fibroblast growth factor 1 (acidic)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibroblast growth factor family
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefibroblast growth factor 13
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Synonyms gene
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Synonyms gene name
- long intergenic non-protein coding RNA 889
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-12-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibroblast growth factor family
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 329-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-11
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR329 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 512-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data