Glutathione Peroxidase (GPx, EC 126.96.36.199) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. BioVision’s Glutathione Peroxidase Assay Kit measures glutathione peroxidase (GPx) activity through a coupled reaction with glutathione reductase (GR). In the assay, GPx reduce Cumene Hydroperoxide, and oxidize GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH is proportionally to GPx activity in the reactions. The decrease of NADPH can be easily measured by absorbance at 340 nm. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with detection sensitivity ~ 0.5 mU/ml of GPx in samples.
• Detection method: Absorbance (340 nm) • Species reactivity: Mammalian • Application: The assay has a detection sensitivity ~ 0.5 mU/ml of GPx in samples.
• Simple procedure; takes ~ 30-40 minutes • Fast and convenient
Kit's other name
• GPx Assay Buffer • NADPH (lyophilized) • Glutathione Reductase • Glutathione (GSH, lyophilized) • Cumene Hydroperoxide • GPx Positive Control (lyophilized)
Maximum time can be stored
Cell and tissue culture supernatants, urine, plasma and serum, erythrocyte lysates, as well as many other biological fluids, etc.
Recombinant human protein purified from E.coli
100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Ammonium sulphate precipitation
For Research Use Only! Not to be used in humans.
The antibody solution should be gently mixed before use.