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Synonyms
RAC-alpha serine/threonine-protein kinase, AKT1, PKB, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene cAkt
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Alternative_names
RAC-alpha serine/threonine-protein kinase, AKT1, PKB, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene cAkt
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Description
One of 3 serine/threonine-protein kinases that regulate many process including metabolism, proliferation, cell survival, growth and angiogenesis.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
>92%
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Assay
SDS-PAGE
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Molecular Weight
60 kDa
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Storage Temp
+4°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Dry powder
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Physical form description
Lyophilized powder
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Reconstitution Instructions
Reconstitute in sterile deionized water to a stock solution of 200 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage.
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Background Information
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity.
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Amino acid sequence
Met 1 - Ala 480
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
CellExp™ Akt1 Protein (His Tagged & Strep Tagged), recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Label
His
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Species
Humans
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Alternative name
H. sapiens CellExp™ v-akt murine thymoma viral oncogene homolog 1 Protein (histidine Tagged & Strep Tagged), H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
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