Human CellExp™ Akt1 Protein (His Tagged & Strep Tagged), human recombinant

  • Catalog number
    7886-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    RAC-alpha serine/threonine-protein kinase, AKT1, PKB, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene cAkt
  • Alternative_names
    RAC-alpha serine/threonine-protein kinase, AKT1, PKB, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene cAkt
  • Description
    One of 3 serine/threonine-protein kinases that regulate many process including metabolism, proliferation, cell survival, growth and angiogenesis.
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    >92%
  • Assay
    SDS-PAGE
  • Molecular Weight
    60 kDa
  • Storage Temp
    +4°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Appearance
    Dry powder
  • Physical form description
    Lyophilized powder
  • Reconstitution Instructions
    Reconstitute in sterile deionized water to a stock solution of 200 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage.
  • Background Information
    AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity.
  • Amino acid sequence
    Met 1 - Ala 480
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Conjugation
    histidine
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    CellExp™   Akt1   Protein   His   Tagged   Strep   Tagged  
  • Short name
    CellExp™ Akt1 Protein (His Tagged & Strep Tagged), recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Label
    His
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ v-akt murine thymoma viral oncogene homolog 1 Protein (histidine Tagged & Strep Tagged), H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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