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Also known as
Vimentin
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Other names
vimentin; Vimentin; vimentin; vimentin; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
VIM; VIM; CTRCT30; N/A
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Gene name synonims
VIM; VIM; CTRCT30; N/A
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Other gene names
VIM; VIM; CTRCT30; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
RV202
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Host organism
Mouse (Mus musculus)
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Species reactivity
Canine, Chicken, Caprine, Hamster, Human (Homo sapiens), Monkey, Mouse (Mus musculus), Rat (Rattus norvegicus), Swine, Zebrafish; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
RV202-HRP reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-HRP is suitable for immunoblotting, immunocytochemistry on cell cultures and cytospins, as well as immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:10.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Each vial contains 1ml HRP-conjugated vimentin monoclonal antibody in PBS containing 0,1% BSA, 0.09% sodium azide. Approximately 100 tests.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius., or in small aliquots the antibody should be stored at -20 degrees Celsius..
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Tested applications
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
