Anti-Mouse Bcl-3, FITC (Clone Ham 150-3.5) (hamster IgG)
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Catalog numberGEN520011
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PricePlease ask
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Size100ug
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Also known asBcl-3
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Other namesB-cell lymphoma 3 protein; B-cell lymphoma 3 protein; B-cell lymphoma 3 protein; BCL-3; proto-oncogene BCL3; B-cell leukemia/lymphoma 3; B-cell lymphoma 3-encoded protein; chronic lymphatic leukemia protein; B-cell CLL/lymphoma 3; Proto-oncogene BCL3
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameBcl-3
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Gene name synonimsN/A
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Other gene namesBCL3; BCL3; BCL4; D19S37; BCL4; D19S37; BCL-3
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ClonalityMonoclonal
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Immunoglobulin isotypeHamster IgG1lambda
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CloneHam 150-3.5
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Host organismHamster
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Species reactivityMouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityBcl-3 (Mouse Bcl-3); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceFITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
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ConcentrationAntibody Concentration: 0.1 miligram per 1 mililiter
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Storage and shippingStore the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid multiple freeze/thaw cycles and prolonged exposure to light
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Tested applicationsImmunohistochemistry (IHC)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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ConjugationAnti-FITC Antibody
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolZNF212, MIR150, GLOD4, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12
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Short nameAnti-Mouse Bcl-3, FITC (Clone Ham 150-3 5) ( IgG)
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Techniqueanti-, Mouse, anti, FITC, IgG, antibody to, antibodies, Fluorescein, IgGs, mouses
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Hostmouse
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IsotypeIgG, IgG
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LabelFITC
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SpeciesHamster, Hamsters, Mouses
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Alternative nameAnti-Anti-Mouse Bcl-3, FITC (Clone Ham 150-3.5) (hamster IgG)
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Alternative techniquemurine, antibodies, fluorescine, immunoglobulins
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Clone nameHam
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Gene info
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Identity
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Gene
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Long gene namezinc finger protein 212
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 150
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene nameglyoxalase domain containing 4
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Synonyms gene
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Synonyms gene name
- chromosome 17 open reading frame 25
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-06-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Glyoxalase domain containing family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 14
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 12
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data