Mouse anti NCAM / CD56
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Catalog numberMUB1300S
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PricePlease ask
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Size1 mL
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CategoryPrimary Antibodies
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Long descriptionNCAM / CD56, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.
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Antibody come fromRNL-1 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with the small cell lung cancer cell line NCI-H82.
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Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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CloneRNL-1
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Antigen antibody binding interactionMouse anti NCAM / CD56 Antibody
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Antibody is raised inMouse
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Antibody s reacts withHuman,Mouse
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityRNL-1 was defined as a cluster I antibody during the Second International Workshop on Small Cell Lung Cancer (SCLC) Antibodies and recognizes the extracellular region of NCAM / CD56.
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Research interestCancer,Cell adhesion,Neurobiology, CD Markers
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ApplicationImmunocytochemistry,Immunohistochemistry (frozen)
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Antibody s suited forRNL-1 is suitable for immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration.
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StorageStore at 4°C, or in small aliquots at -20°C.
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Relevant referencesBroers, J. L., Mijnheere, E. P., Rot, M. K., Schaart, G., Sijlmans, A., Boerman, O. C., and Ramaekers, F. C. (1991). Novel antigens characteristic of neuroendocrine malignancies, Cancer 67, 619-33. Mijnheere, E. P., Boerman, O. C., Broers, J. L., Klein Rot, M., Vooijs, G. P., and Ramaekers, F. C. (1991). Immunotargeting of Human small cell lung cancer xenografts in athymic mice using a monoclonal antibody (RNL-1) anti a neuroendocrine- related antigen, Br J Cancer Suppl 14, 78-81. Boerman, O. C., Mijnheere, E. P., Broers, J. L., Vooijs, G. P., and Ramaekers, F. C. (1991). Biodistribution of a monoclonal antibody (RNL-1) anti the neural cell adhesion molecule (NCAM) in athymic mice bearing Human small-cell lung-cancer xenografts, Int J Cancer 48, 457-62. Mijnheere, E. P., Boerman, O. C., Poels, L. G., Slobbe, R. L., van Eys, G. J. J. M., and Ramaekers, F. C. S. (1996). Pharmacokinetics, biodistribution and dosimetry of anti-NCAM monoclonal antibody RNL-1 and its fragments in experimental lung cancer, Journal of Experimental and Clinical Cancer Research 15, 11-18. Hens, J., Nuydens, R., Geerts, H., Senden, N. H., Van de Ven, W. J., Roebroek, A. J., van de Velde, H. J., Ramaekers, F. C., and Broers, J. L. (1998). Neuronal differentiation is accompanied by NSP-C expression, Cell Tissue Res 292, 229-37.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolNCAM1, L1CAM
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Short nameMouse anti NCAM / CD56
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TechniqueMouse, anti, antibody to, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to NCAM / CD56
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameneural cell adhesion molecule 1
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Fibronectin type III domain containing
- CD molecules
- Ig-like cell adhesion molecule family
- I-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameL1 cell adhesion molecule
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Synonyms gene
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Synonyms gene name
- antigen identified by monoclonal antibody R1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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RefSeq identity
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Classification
- Ig-like cell adhesion molecule family
- I-set domain containing
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data