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Anti-KDEL (ER Marker) (Polyclonal), ALEXA Fluor 594

Anti-KDEL (ER Marker) (Polyclonal), ALEXA Fluor 594 is available 1 time from Bioss polyclonal antibodies labs

GENTObs-6940R-A594 | Anti-KDEL (ER Marker) (Polyclonal), ALEXA Fluor 594 size: 100 microliters | 552.81 USD

Catalog number GENTObs-6940R-A594
Price552.81 USD
Size 100 microliters
MeSH Data
Concept
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Additional information
Modification No modification has been applied to this antibody
Clonality Polyclonal Antibody
Other name Anti-KDEL (ER Marker) Polyclonal
Host organism Rabbit (Oryctolagus cuniculus)
Background information Endoplasmic reticulum Marker. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
Also known as KDEL (ER Marker) Polyclonal Antibody
Swiss Prot N/A
Specificity This antibody reacts specifically with KDEL (ER Marker)
Modification site None
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Conjugated with ALEXA FLUOR® 594
Gene ID N/A
Clone Polyclonal Antibodies
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Synonyms KDEL; Lys Asp Glu Leu antibody; GRP 78; GRP 94; KDEL1_HUMAN.
Applications IF(IHC-P)
Subcellular locations N/A
Concentration 1ug per 1ul
Purification method Purified by Protein A.
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Type Conjugated Primary Antibody
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus)
Excitation emission 590nm/617nm
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Target Protein/Peptide KDEL ER Marker
Antigen Source KLH conjugated synthetic peptide KDEL
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Gene targetKDEL
Short name Anti-KDEL (ER ) (Polyclonal)
Technique Polyclonal, anti
Label ALEXA FLUOR 594
Alternative name antibody to-KDEL (ER detection labelled) (polyclonal), ALEXA Fluor 594
Alternative technique polyclonals
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