p3*Flag-CMV-14-ARID3B Plasmid
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Catalog numberPVTB00925-2a
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Price381.56 USD
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Size2 ug
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PropertiesAn anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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KitPlasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbolARID3B, PIRC60, IGHV3OR16-14, SNORD114-14
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Short namep3* -CMV-14-ARID3B Plasmid
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Techniqueplasmid, plasmids in 1
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LabelFlag
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Alternative namep3*Flag-cytomegalovirus-14-ARID3B Plasmid
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Alternative techniqueplasmids
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Viruscmv
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Gene info
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Identity
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Gene
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Long gene nameAT-rich interaction domain 3B
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Synonyms gene name
- AT rich interactive domain 3B (BRIGHT- like)
- AT rich interactive domain 3B (BRIGHT-like)
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Synonyms
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Locus
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Discovery year2004-01-28
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Entrez gene record
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RefSeq identity
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Classification
- AT-rich interaction domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 60
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-14 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-14
- immunoglobulin heavy variable 3/OR16-14 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-14
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box