Rabbit Anti-Human MAP4K5 (pSer174) Polyclonal: ATTO 655
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Catalog numberSPC-1002D-A655
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Price:
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Ser174 of human KHS1 (AA171-177)
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Antibody s targetHuman MAP4K5 (pSer174)
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Antibody s full descriptionRabbit Anti-Human MAP4K5 (pSer174) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameKHS Antibody, Kinase homologous to SPS1/STE20 Antibody, MAPK/ERK kinase kinase kinase 5 Antibody, MEK kinase kinase 5 Antibody, MEKKK 5 Antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 95.04 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1002 was sufficient for detection of MAP4K5 (pSer174) in 10 µg of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificityUbiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate
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Scientific contextMay play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_006566
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Gene number11183
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Protein numberQ9Y4K4
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolMAP4K5
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Short nameRabbit Anti- MAP4K5 (pSer174) Polyclonal: ATTO 655
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 655
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman mitogen-activated protein kinase kinase kinase kinase 5 (pSer174) polyclonal: ATTO 655
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Alternative to gene targetmitogen-activated protein kinase kinase kinase kinase 5, GCKR and KHS and KHS1 and MAPKKKK5, MAP4K5 and IDBG-6029 and ENSG00000012983 and 11183, transferase activity, Plasma membranes, Map4k5 and IDBG-144865 and ENSMUSG00000034761 and 399510, MAP4K5 and IDBG-646448 and ENSBTAG00000014792 and 781335
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Gene info
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Identity
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Gene
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Long gene namemitogen-activated protein kinase kinase kinase kinase 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitogen-activated protein kinase kinase kinase kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data