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Synonyms
MSLN, Mesothelin, MPF, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor
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Alternative_names
MSLN, Mesothelin, MPF, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor
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Description
Membrane-anchored forms may play a role in cellular adhesion.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Human Mesothelin, Fc Tag at 0.5 ug/mL (100 uL/well) can bind Anti-Human MSLN Mab with a linear range of 0.8-3 ng/mL
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Binding Capacity
Immobilized Human Mesothelin, Fc Tag at 0.5 ug/mL (100 uL/well) can bind Anti-Human MSLN Mab with a linear range of 0.8-3 ng/mL
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Molecular Weight
33.0 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to a concentration of 50 ug/ml.
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Background Information
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MSLN
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Short name
CellExp™ Mesothelin / MSLN, Fc Tag, Recombinant
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Technique
Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
Fc Tag
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Species
Humans
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Alternative name
H. sapiens CellExp™ Mesothelin / mesothelin, fragment c detection labelled, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
mesothelin, MPF and SMRP, MSLN and IDBG-8270 and ENSG00000102854 and 10232, protein binding, Extracellular, Msln and IDBG-152832 and ENSMUSG00000063011 and 56047, MSLN and IDBG-634054 and ENSBTAG00000000177 and 516237
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