This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-TUCAN polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5 BSA and 0.01% thimerosal.
Alternative names of antibody target:
DACAR , NDPP1 , TUCAN , CARDINAL , KIAA0955 , MGC57162 , DKFZp779L0366
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 47 kDa TUCAN in samples from human, mouse, and rat origins. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysates can be used as positive controls.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Human, mouse, rat
Clonality of antibody:
Synthetic peptide surrounding amino acid 420 of human TUCAN
How to handle this antibody:
The antibody solution should be gently mixed before use.
TUCAN (Tumor Up-regulated Card-containing Antagonist of Caspase-Nine) is a CARD (Caspase-Associated Recruitment Domains) containing protein that binds and suppresses activation of pro-Caspase-9 and found to be overexpressed in some types of cancers. TUCAN binds specifically to pro-caspase-9 and interferes with its interaction with Apaf-1, thereby suppressing apoptosis signaling in the mitochondrial/cytochrome c pathway.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
antibody to-TUCAN/caspase recruitment domain family, member 8 (Antibody to)
Alternative to gene target
caspase recruitment domain family, member 8, CARDINAL and DACAR and DAKAR and NDPP and NDPP1 and TUCAN, CARD8 and IDBG-60120 and ENSG00000105483 and 22900, protein homodimerization activity, nuclei