PI3K (p85⍺) Protein

Synonyms

PI3K (p85⍺) Protein, PI3K, PI3 kinase
Alternative_names

PI3K (p85⍺) Protein, PI3K, PI3 kinase
Description

Intracellular signal transducer enzyme
Recombinant

Yes
Source

Baculovirus (Sf9 insect cells)
Purity by SDS PAGE

≥95%
Assay

SDS-PAGE
Molecular Weight

86.0 kDa
Storage Temp

-80°C
Shipping

dry ice
Shelf Life

12 months
Concentration

0.2 mg/ml
Appearance

Liquid
Physical form description

Recombinant protein in storage buffer (50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 2 mM DTT, 150 mM imidazole, 0.1 mM PMSF, 25 % glycerol.).
Background Information

The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85αmodulates the interaction between PI3K and platelet-derived growth factor receptor (1). Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85α regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS) (2).
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet

Identity

HGNC:8972
Gene

PIK3C2B
Long gene name

phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Synonyms gene name
- phosphoinositide-3-kinase, class 2, beta polypeptide
- phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta
Synonyms
- C2-PI3K
- PI3K-C2beta
GenBank acession
- Y11312
Locus

1q32.1
Discovery year

1998-05-21
Entrez gene record

5287
Pubmed identfication
- 9144573
- 9830063
RefSeq identity
- NM_002646
Classification
- Phosphatidylinositol 3-kinase subunits
VEGA ID

OTTHUMG00000036101

Identity

HGNC:8975
Gene

PIK3CA
Long gene name

phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms gene name
- phosphoinositide-3-kinase, catalytic, alpha polypeptide
- phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
Synonyms
- PI3K
Locus

3q26.32
Discovery year

1994-07-15
Entrez gene record

5290
Pubmed identfication
- 1322797
Classification
- Phosphatidylinositol 3-kinase subunits
VEGA ID

OTTHUMG00000157311
Locus Specific Databases
- LRG_310

Identity

HGNC:8971
Gene

PIK3C2A
Long gene name

phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms gene name
- phosphoinositide-3-kinase, class 2, alpha polypeptide
- phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha
Synonyms
- PI3K-C2alpha
GenBank acession
- Y13367
Locus

11p15.1
Discovery year

1998-05-21
Entrez gene record

5286
Pubmed identfication
- 9337861
RefSeq identity
- NM_002645
Classification
- Phosphatidylinositol 3-kinase subunits
VEGA ID

OTTHUMG00000166036