Complement Factor C3c : FITC
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Catalog number
C3C001-F
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Price
Please ask
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Size
1 mL
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Clone
NA
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Species Reactivity
Human
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Product Types
Primary Antibodies
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Positive Control
Skin (basal membrane) in bullous pemphigoid
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Immunogen
Purified Complement 3 from human serum
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Working Concentration
(liquid conc.) 1:50 - 1:100
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Concentration
10 mg/ml
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Incubation Time
NA
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Application
IHC (F); The specified dilution has to be evaluated individually for optimal results.
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Storage
2-8°C, protected from light
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UniProt
P01024-CO3_HUMAN
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Properties
This Nordic MUbio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Nordic MUbio FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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