EZClick™ TUNEL – in situ DNA Fragmentation/Apoptosis Assay Kit
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Catalog number
K191-100
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Price
Please ask
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Size
100 assays
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Description
Non-antibody based assay for the detection of apoptotic cells
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Detection Method
Flow cytometer w/lasers capable of excitation at 488 and 530/590 nm emission filters respectively
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Species Reactivity
All
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Applications
Detection of DNA fragmentation during apoptosis and programmed cell death,Evaluating effects of anti-cancer drugs and genotoxic agents on DNA structure,Screening for genotoxic compounds and effectors of DNA integrity and cellular changes,In-situ studies of apoptosis and programmed cell death
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Sample Type
Cell culture
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Features Benefits
Does not require antibodies, avoiding lengthy incubation times and does not require washes,Specific, sensitive,Compatible with Flow Cytometry and Fluorescence Microscopy instruments
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Apoptosis is a controlled phenomenon in which apoptotic cells are characterized by changes in nucleus (i.e. chromatin condensation, DNA fragmentation), cytoplasm shrinkage and production of apoptotic bodies. These are subsequently phagocytosed by macrophages, parenchymal cells, or neoplastic cells. DNA strand breaks can be analyzed by TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End-Labeling) assays. BioVision’s EZClickTM TUNEL-DNA fragmentation/Apoptosis Assay utilizes modified EdUTP nucleotides which are incorporated at the 3’-OH ends of fragmented DNA by Terminal deoxynucleotidyl transferase (TdT) enzyme and detected based on a click reaction. This assay enables easy delivery and incorporation of EdUTP coupled with high selectivity of detection of DNA damage. The assay is performed under mild reaction conditions which preserve cell morphology, thus enabling the identification of damaged DNA by FACS or fluorescence microscopy. Our assay is fast and capable of detecting a higher percentage of apoptotic cells than the antibody based methods. The kit contains sufficient reagents to detect total/fragmented DNA in apoptotic cells in a 1 X 96-well plate or on 50 cover slips.
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Additional description
This 1 is suited for programmed cell-death studies.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
EZClick™ TUNEL – in situ DNA Fragmentation/Apoptosis Assay Kit
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Technique
apoptosis, Assay, dna, apoptosis pathway, assays
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Host
Assay
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Alternative name
EZClick™ TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling – in situ Desoxyribonucleic acid Fragmentation/programmed cellular destruction test reagent
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Alternative technique
celldeath, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A technique that labels specific sequences in whole chromosomes by in situ DNA chain elongation or PCR (polymerase chain reaction).
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Tree numbers
- E01.370.225.500.620.670.325.680
- E01.370.225.750.600.670.325.680
- E05.200.500.620.670.325.680
- E05.200.750.600.670.325.680
- E05.393.620.500.650
- E05.393.661.475.680
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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