Anti-Pyruvate Dehydrogenase E1-α subunit Antibody (3H2-F8-B5)

• Catalog number
  A1323-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Pyruvate dehydrogenase E1 component subunit alpha
• Target Alternative Name
  Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1, PHE1A
• Antibody Type
  Monoclonal
• Clone
  3H2-F8-B5
• Species Reactivities
  Human, Mouse
• Immunogen Sequence
  Recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli
• Accession
  P08559
• Gene Id
  5160
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Purification
  Affinity purified
• Application
  WB, IF
• Positive Control
  WB: 293, 1299, mEsc and Tc1 cell lysates; IF: HeLa cells
• Application And Usages
  WB; 1:1000, IF; 1:100
• Description
  The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Pyruvate   Dehydrogenase   E1-α   subunit   3H2-F8-B5  
• Gene symbol
  RTKN, MAGEB5, PCDHGB5, CYB5R4, F8, EIF3M
• Short name
  Anti-Pyruvate Dehydrogenase E1-α subunit Antibody (3H2-F8-B5)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG1
• Alternative name
  Antibody toPyruvate Dehydrogenase E1-α functionnal sequence (antibody to-) (3H2-coagulation factor VIII, procoagulant component-B5)
• Alternative technique
  antibodies
• Alternative to gene target
  coagulation factor VIII, procoagulant component, AHF and DXS1253E and F8B and F8C and FVIII and HEMA, F8 and IDBG-92134 and ENSG00000185010 and 2157, oxidoreductase activity, Extracellular, F8 and IDBG-157124 and ENSMUSG00000031196 and 14069, F8 and IDBG-632125 and ENSBTAG00000010726 and 100271720

Gene info

• Identity
  HGNC:10466
• Gene
  RTKN
• Long gene name
  rhotekin
• Synonyms
  • B5
• GenBank acession
  • AF049227
• Locus
  2p13.1
• Discovery year
  1996-11-15
• Entrez gene record
  6242
• Pubmed identfication
  • 9073523
  • 10940294
• RefSeq identity
  • NM_001015055
• Classification
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000129955

Gene info

• Identity
  HGNC:23795
• Gene
  MAGEB5
• Long gene name
  MAGE family member B5
• Synonyms gene name
  • melanoma antigen family B5
• Synonyms
  • MAGE-B5
  • CT3.3
• Synonyms name
  • cancer/testis antigen family 3, member 3
• GenBank acession
  • AF333705
• Locus
  Xp21.3
• Discovery year
  2003-12-10
• Entrez gene record
  347541
• Pubmed identfication
  • 10861452
• RefSeq identity
  • NM_001271752
• Classification
  • MAGE family
• VEGA ID
  OTTHUMG00000021288
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:8712
• Gene
  PCDHGB5
• Long gene name
  protocadherin gamma subfamily B, 5
• Synonyms
  • PCDH-GAMMA-B5
• GenBank acession
  • AF152521
• Locus
  5q31
• Discovery year
  2000-06-28
• Entrez gene record
  56101
• Pubmed identfication
  • 10380929
• RefSeq identity
  • NM_018925
• Classification
  • Clustered protocadherins
• VEGA ID
  OTTHUMG00000188231

Gene info

• Identity
  HGNC:20147
• Gene
  CYB5R4
• Long gene name
  cytochrome b5 reductase 4
• Synonyms gene
  • NCB5OR
• Synonyms gene name
  • NADPH cytochrome B5 oxidoreductase
• Synonyms
  • b5+b5R
  • dJ676J13.1
• GenBank acession
  • AF169803
• Locus
  6q14.2
• Discovery year
  2003-08-27
• Entrez gene record
  51167
• Pubmed identfication
  • 10611283
• RefSeq identity
  • NM_016230
• VEGA ID
  OTTHUMG00000015118

Gene info

• Identity
  HGNC:3546
• Gene
  F8
• Long gene name
  coagulation factor VIII
• Synonyms gene
  • F8C
• Synonyms gene name
  • coagulation factor VIII, procoagulant component
• Synonyms
  • FVIII
  • DXS1253E
  • HEMA
• Synonyms name
  • Factor VIIIF8B
  • hemophilia A
• GenBank acession
  • M90707
• Locus
  Xq28
• Discovery year
  2001-06-22
• Entrez gene record
  2157
• Pubmed identfication
  • 6438528
  • 3935400
• VEGA ID
  OTTHUMG00000022688
• Locus Specific Databases
  • HAMSTeRS: The Haemophilia A Mutation, Structure, Test & Resource Site
  • Global Variome shared LOVD
  • LRG_555

Gene info

• Identity
  HGNC:24460
• Gene
  EIF3M
• Long gene name
  eukaryotic translation initiation factor 3 subunit M
• Synonyms gene
  • PCID1
• Synonyms gene name
  • PCI domain containing 1 (herpesvirus entry mediator)
  • eukaryotic translation initiation factor 3, subunit M
• Synonyms
  • hfl-B5
  • FLJ29030
  • GA17
  • TANGO7
• Synonyms name
  • transport and golgi organization 7 homolog (Drosophila)
• GenBank acession
  • AK131064
• Locus
  11p13
• Discovery year
  2006-03-31
• Entrez gene record
  10480
• Pubmed identfication
  • 15919898
  • 15919899
• RefSeq identity
  • NM_006360
• Classification
  • Armadillo like helical domain containing
  • Eukaryotic translation initiation factor 3
• VEGA ID
  OTTHUMG00000166258

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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