Human CellExp™ CD19, Human recombinant
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Catalog number9246-25
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PricePlease ask
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Size25 µg
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SynonymsCD19, B4, CVID3, MGC12802
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Alternative_namesCD19, B4, CVID3, MGC12803
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DescriptionB-lymphocyte antigen CD20
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RecombinantYes
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SourceHEK 293 cells
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Purity by SDS PAGE>95%
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AssaySDS-PAGE
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Purity>95%
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Endotoxin Level< 1 EU/ug
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Biological activityImmobilized FMC63 mAb at 2ug/mL(100uL/well) can bind Human CD19 (20-291), His Tag with a linear range of 0.04-0.6 ug/mL.
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Binding CapacityImmobilized FMC63 mAb at 2ug/mL(100uL/well) can bind Human CD19 (20-291), His Tag with a linear range of 0.04-0.6 ug/mL.
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Molecular WeightThis protein is fused with a 6× His tag at N-terminus and has a calculated MW of 31.6 kDa.
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized
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Reconstitution InstructionsReconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml
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Background InformationB-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCD19
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Short nameCellExp™ CD19, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ CD19 molecule, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetCD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
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Gene info
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Identity
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Gene
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Long gene nameCD19 molecule
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Synonyms gene name
- CD19 antigen
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Locus
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Discovery year1991-06-04
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
- Minor histocompatibility antigens
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data