Asparagine Assay Kit (Colorimetric)

• Catalog number
  K736-100
• Price
  Please ask
• Size
  100 assays

• Description
  First assay in the market that measures Asparagine in biological Samples
• Summary
  • Detection method- Fluorescence (Ex/Em= 535/587 nm) • Species reactivity- Eurkayotes and Prokaryotes
• Detection Method
  Fluorescence (Ex/Em= 535/587 nm)
• Species Reactivity
  Eukaryotes and Prokaryotes
• Applications
  Measurement of Asparagine in biological samples
• Sample Type
  Cell and tissue culture supernatants, CSF, plasma, and serum
• Features Benefits
  • Simple procedure; takes ~ 60 minutes • Fast and convenient • Kit contains the necessary reagents for accurate measurement of Asparagine
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Asparagine is one of the 20 naturally-occuring amino acids used by all organisms in the biosynthesis of proteins. In humans, it is considered a non-essential amino acid, as it can be synthesized in vivo from aspartate and glutamine. In addition to its role as a protein building block, asparagine is vital for pre- and postnatal neuronal development. Congenital deficiency of the enzyme Asparagine Synthetase (EC 6.3.5.4) results in microcephaly, intractable seizures and severe neurodevelopmental disabilities. In contrast, depletion of asparagine levels is employed as a treatment strategy for certain cancers, particularly acute lymphoblastic leukemia (ALL) and other hematopoietic cancers. Lymphoblasts are incapable of synthesizing asparagine de novo and are thus dependent on exogenous sources. Treatment with the bacterial enzyme Asparaginase (EC 3.5.1.1) deprives malignant cells of the circulating plasma asparagine needed for rapid growth. BioVision’s Asparagine Assay Kit allows for highly sensitive quantification of L-asparagine levels in biological fluids and cultured cells. In the assay, L-asparagine is hydrolysed to generate aspartate, which is converted to pyruvate and subsequently reacts with a fluorogenic probe to form a stable fluorophore (Ex/Em = 538/587 nm) via a series of coupled enzymatic reactions. The assay is not affected by physiological concentrations of other common amino acids and can detect asparagine levels down to 5 μM in samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Asparagine   Kit  
• Short name
  Asparagine Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Asparagine test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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