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Synonyms
PVRL1, Nectin-1, CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12
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Alternative_names
PVRL1, Nectin-1, CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12
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Description
Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Nectin-3 /PVRL3 at 1 ug/ml (100 ul/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.25-1000 ng/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Nectin-3 /PVRL3 at 1 ug/ml (100 ul/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.25-1000 ng/ml.
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Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 34.8 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 45-60 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Poliovirus receptor-related protein 1 (PVRL1) is also known as Herpes virus entry mediator C (HveC), Herpesvirus Ig-like receptor (HIgR), Nectin-1, CD antigen CD111, PVRL1 / Nectin-1 / CD111 belongs to the nectin family. PVRL1 / Nectin-1 is a membrane protein with three extracellular immunoglobulin domains, a single transmembrane helix and a cytoplasmic tail. The protein can mediate Ca2+-independent cellular adhesion further characterizing it as IgSF cell adhesion molecule (IgSF CAM). PVRL1 is adhesion molecule found in a wide range of tissues. PVRL1 / Nectin-1 promote cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NECTIN1
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Short name
CellExp™ CD111 / Nectin-1 / PVRL1, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ CD111 / Nectin-1 / poliovirus receptor-related 1 (herpesvirus entry mediator C), H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
poliovirus receptor-related 1 (herpesvirus entry mediator C), CD111 and CLPED1 and ED4 and HIgR and HV1S and HVEC and nectin-1 and OFC7 and PRR and PRR1 and PVRR and PVRR1 and SK-12, PVRL1 and IDBG-74533 and ENSG00000110400 and 5818, cell adhesion molecule binding, Extracellular, Pvrl1 and IDBG-155297 and ENSMUSG00000032012 and 58235, PVRL1 and IDBG-631966 and ENSBTAG00000015939 and 533492
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