Phospho-PKC Antibody

• Catalog number
  3451-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Phospho-PKC
• Target Alternative Name
  Protein kinase C
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic phospho-peptide surrounding amino acid Ser660 of human PKC beta II
• Accession
  NP_002729
• Gene Id
  5579
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  The antibody can be used in Western blotting (1-2 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes several Phosphorylated PKC (Ser660) isoforms of human, mouse, and rat origins. Reactivity to other species has not been tested.
• Description
  PKC (Protein kinase C) is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Phospho-PKC  
• Gene symbol
  PRKCH, PRKD1, PRRT2, PRKDC
• Short name
  Phospho-PKC Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  phosphorilated-PKC (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9403
• Gene
  PRKCH
• Long gene name
  protein kinase C eta
• Synonyms gene
  • PRKCL
• Synonyms gene name
  • protein kinase C, eta
• Synonyms
  • PKC-L
  • PKCL
• GenBank acession
  • M55284
• Locus
  14q23.1
• Discovery year
  1992-06-26
• Entrez gene record
  5583
• Pubmed identfication
  • 1986216
  • 1545821
• RefSeq identity
  • NM_006255
• Classification
  • C2 domain containing protein kinases
  • AGC family kinases
• VEGA ID
  OTTHUMG00000152341

Gene info

• Identity
  HGNC:9407
• Gene
  PRKD1
• Long gene name
  protein kinase D1
• Synonyms gene
  • PRKCM
• Synonyms gene name
  • protein kinase C, mu
• Synonyms
  • PKCM
  • PKD
  • PKC-mu
• Locus
  14q12
• Discovery year
  1994-05-16
• Entrez gene record
  5587
• Pubmed identfication
  • 8119958
  • 10965134
• RefSeq identity
  • NM_002742
• Classification
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000140203

Gene info

• Identity
  HGNC:30500
• Gene
  PRRT2
• Long gene name
  proline rich transmembrane protein 2
• Synonyms gene
  • ICCA
  • DYT10
• Synonyms gene name
  • infantile convulsions and paroxysmal choreoathetosis
  • dystonia 10
  • proline-rich transmembrane protein 2
• Synonyms
  • FLJ25513
  • DKFZp547J199
  • IFITMD1
  • FICCA
  • DSPB3
  • PKC
  • EKD1
• Synonyms name
  • interferon induced transmembrane protein domain containing 1
  • dispanin subfamily B member 3
• GenBank acession
  • BC011405
• Locus
  16p11.2
• Discovery year
  2005-11-25
• Entrez gene record
  112476
• Pubmed identfication
  • 22101681
  • 22243967
  • 29346777
• RefSeq identity
  • NM_145239
• Classification
  • interferon induced transmembrane protein domain containing
• VEGA ID
  OTTHUMG00000177142

Gene info

• Identity
  HGNC:9413
• Gene
  PRKDC
• Long gene name
  protein kinase, DNA-activated, catalytic subunit
• Synonyms gene
  • HYRC
  • HYRC1
• Synonyms gene name
  • protein kinase, DNA-activated, catalytic polypeptide
• Synonyms
  • DNPK1
  • p350
  • DNAPK
  • XRCC7
  • DNA-PKcs
  • DNAPKc
  • DNA-PKC
  • p460
• Synonyms name
  • DNA-dependent protein kinase catalytic subunit
• Locus
  8q11.21
• Discovery year
  1993-11-09
• Entrez gene record
  5591
• Pubmed identfication
  • 7638222
• RefSeq identity
  • NM_001081640
• Classification
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164239
• Locus Specific Databases
  • LRG_162

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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