Rat HRH4(Histamine Receptor H4) EIA Kit
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Catalog number
E-EL-R2556-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat HRH4. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HRH4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HRH4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HRH4. You can calculate the concentration of Rat HRH4 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.19ng/mL
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Detection Range
0.31~20ng/mL
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Target Name
HRH4
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UNIProt ID
Q91ZY1
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat HRH4(Histamine Receptor H4) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
HRH4
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Short name
HRH4(Histamine Receptor H4) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rats
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Alternative name
Rat histamine receptor H4(Histamine Receptor H4) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
histamine receptor H4, HRH4 and IDBG-1754 and ENSG00000134489 and 59340, histamine receptor activity, Plasma membranes, Hrh4 and IDBG-129289 and ENSMUSG00000037346 and 225192, HRH4 and IDBG-643833 and ENSBTAG00000020467 and 783354
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Gene info
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Identity
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Gene
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Long gene name
histamine receptor H4
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Synonyms
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GenBank acession
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Locus
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Discovery year
2002-11-26
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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