Mouse Anti-CD3, conjugated to PE Antibody

• Catalog number
  GEN570365
• Price
  Please ask
• Size
  2 ml

• Also known as
  CD3
• Other names
  cd3; CD3gamma/delta; N/A; N/A; CD3gamma/delta
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  CD3
• Gene name synonims
  N/A
• Other gene names
  N/A; cd3g; N/A; N/A
• Clonality
  Monoclonal antibody
• Immunoglobulin isotype
  IgG1
• Clone
  UCHT1
• Host organism
  Mouse (Mus musculus)
• Species reactivity
  Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  The CD3 mAb (clone UCHT1) recognizes cytoplasmic CD3 epsilon in precursor T-cells and cytoplasmic and surface CD3 epsilon in mature T-lymphocytes. The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).In practice, 50 ul of leukocytes containing 107 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Purified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3
• Concentration
  N/A
• Storage and shipping
  the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody. To enchance the antibody's stability sodium azide has been added. Keep the antibody at +4 degrees Celsius and do NOT freeze it. the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light. Please refer to the expiry date printed on the vial. The use of the reagent after the expiration date is not recommended.
• Tested applications
  Flow Cytometry (FC/FACS), Indirect Immunofluorescence (IF)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  CD3   conjugated  
• Short name
  Mouse Anti-CD3, conjugated PE Antibody
• Technique
  Antibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
• Host
  mouse
• Label
  PE
• Species
  Mouse, Mouses
• Alternative name
  Anti-Mouse anti CD3, conjugated to PE
• Alternative technique
  antibodies, murine

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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