Anti-CKLF (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-2394R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  CKLF
• Specificity
  This antibody reacts specifically with CKLF
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Transmembrane
• Antigen Source
  KLH conjugated synthetic peptide derived from human CKLF
• Gene ID
  51192
• Swiss Prot
  Q9UBR5
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  May play an important role in inflammation and regeneration of skeletal muscle. Partly inhibited by interleukin 10. Isoform 1 has chemotactic response in rat monocytes, neutrophils, and lymphocytes.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  C32; CKLF1; CKLF2; CKLF3; CKLF4; UCK-1; HSPC224; Chemokine-like factor; CKLF; UNQ410/PRO772
• Also known as
  CKLF Polyclonal Antibody
• Other name
  Anti-CKLF Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  CKLF  
• Gene symbol
  CKLF-CMTM1, KLF5, CKLF
• Short name
  Anti-CKLF
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  CKLF Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies
• Alternative to gene target
  chemokine-like factor, CKLF and IDBG-281643 and ENSG00000217555 and 51192, chemokine activity, Extracellular, Cklf and IDBG-185208 and ENSMUSG00000054400 and 75458, CKLF and IDBG-633073 and ENSBTAG00000038858 and 525286

Gene info

• Identity
  HGNC:39977
• Gene
  CKLF-CMTM1
• Long gene name
  CKLF-CMTM1 readthrough
• Locus
  16q21
• Discovery year
  2011-04-19
• Entrez gene record
  100529251
• VEGA ID
  OTTHUMG00000167106

Gene info

• Identity
  HGNC:6349
• Gene
  KLF5
• Long gene name
  Kruppel like factor 5
• Synonyms gene
  • BTEB2
• Synonyms gene name
  • Kruppel-like factor 5 (intestinal)
• Synonyms
  • IKLF
  • CKLF
• Synonyms name
  • (intestinal Kruppel-like factor
• GenBank acession
  • D14520
• Locus
  13q22.1
• Discovery year
  2000-03-06
• Entrez gene record
  688
• Pubmed identfication
  • 8479902
  • 9973612
• Classification
  • Kruppel like factors
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000017074

Gene info

• Identity
  HGNC:13253
• Gene
  CKLF
• Long gene name
  chemokine like factor
• Synonyms gene
  • CKLF1
• Synonyms gene name
  • chemokine-like factor 1
• Synonyms
  • UCK-1
  • CKLF3
  • CKLF4
  • HSPC224
  • C32
• GenBank acession
  • AF096895
• Locus
  16q21
• Discovery year
  2001-08-23
• Entrez gene record
  51192
• Pubmed identfication
  • 11042152
  • 11415443
• RefSeq identity
  • NM_016326
• VEGA ID
  OTTHUMG00000137504

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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