This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Alternative names of antibody target:
Apolipoprotein E3, APOE 3, APOE3, APOE-3
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Recombinant ApoA1 (Cat. # 4696-100) can be used as a positive control. The antibody recognizes ~34 kDa of human ApoE3. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
Recombinant human ApoE3
How to handle this antibody:
The antibody solution should be gently mixed before use.
ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. It also binds with the LDL receptor (apo B/E). Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.