Oxaloacetate Colorimetric/Fluorometric Assay Kit
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Catalog number
K659-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
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Sample Type
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of OAA levels
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Oxaloacetate (OAA, HOOC-CO-CH₂-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. BioVision's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (λmax= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Oxaloacetate Colorimetric/Fluorometric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Oxaloacetate colorimetry/fluorescence test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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