Recombinant Human Nicastrin / NCSTN Protein (His tag) 20µg
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Catalog number
PKSH031225-20µg
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Price
Please ask
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Size
20µg
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Synonym
APH2;ATAG1874;KIAA0253;NCSTN;RP11-517F10.1
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Activity
NA
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Sequence
Met 1-Glu 669
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Fusion tag
C-His
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Accession
NP_056146.1
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
＞93 % as determined by SDS-PAGE
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Endotoxin
＜1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
72.4 kDa
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AP Mol Mass
110-120 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Reconstitution
Please refer to it for detailed information.
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Background
Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NCSTN
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Short name
Recombinant Nicastrin / NCSTN Protein (His tag) 20µg
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens Nicastrin / nicastrin Protein (histidine detection labelled) 20µg
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Alternative technique
rec
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Alternative to gene target
nicastrin, ATAG1874, NCSTN and IDBG-104034 and ENSG00000162736 and 23385, peptidase activity, Plasma membranes, Ncstn and IDBG-204941 and ENSMUSG00000003458 and 59287, BT.48812 and IDBG-630578 and ENSBTAG00000004334 and 514929
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Gene info
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Identity
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Gene
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Long gene name
nicastrin
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Synonyms
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GenBank acession
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Locus
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Discovery year
2005-02-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
Product images
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