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Alternative name1
Anti-RABBIT ANTI MOUSE IgG2a:FITC
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Alternative name2
RABBIT ANTI Murine IgG2a:FITC
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Alternative name3
Lapin ANTI Mus musculus IgG2a:FITC
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Alternative name4
IgG2a
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Alternative name5
N/A
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Other names
Ig gamma-2A chain C region secreted form; Ig gamma-2A chain C region secreted form; N/A; N/A; B allele
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Gene name
IgG2a
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
Polyclonal IgG
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
Mouse
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Specificity and cross reactivity
IgG2a This item recognises murine IgG2a and shows
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Purification method
N/A
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Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilised)
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Concentration
1.0 mg/ml
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Storage and shipping
Prior to reconstitution store at +Store productone at +4 degrees Celsius.. After reconstitution store at +Store productone at +4 degrees Celsius. or productone should be stored at -20 degrees Celsius. if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. productone is photosensitive and should be protected from light. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of reconstitution
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Tested for
Immunofluorescence (IF)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
