Iron Colorimetric Assay Kit
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Catalog number
K390-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Absorbance (593 nm) • Species reactivity- Mammalian • Applications- The kit can detect 8 µM to 400 µM iron concentration in various samples.
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Sample Type
Cell and tissue lysates, culture media, urine, and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • A specific chelate chemical is included in the buffer to block copper ion (Cu²ᶧ) interference.
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Storage Conditions
+4°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Iron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase. BioVision's Iron Assay Kit provides a simple convenient means of measuring Ferrous and/or Ferric ion in sample. In the assay, ferric carrier protein will dissociate ferric into solution in the presence of acid buffer. After reduction to the ferrous form (Fe²ᶧ), iron reacts with Ferene S to produce a stable colored complex and give absorbance at 593 nm. A specific chelate chemical is included in the buffer to block copper ion (Cu²ᶧ) interference. The kit measures iron in the linear range of 0.4 to 20 nmol in 50 µl sample, or 8 µM to 400 µM iron concentration in various samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Iron Colorimetric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Iron colorimetry test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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