PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)
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Catalog number
K751-100
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Price
Please ask
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Size
100 assays
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Description
The most sensitive kit on the market
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Summary
• Detection method- Fluorescence (Ex/Em = 535/587 nm) • Application- Measurement of G6PDH activity in various tissues and cells - Evaluation of pentose phosphate pathway
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Detection Method
Fluorescence (Ex/Em = 535/587 nm)
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Applications
• Measurement of G6PDH activity in various tissues and cells • Evaluation of pentose phosphate pathway
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Sample Type
• Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: adherent or suspension cells • Plant tissues
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Features Benefits
• Rapid & simple • High-throughput adaptable • Can detect as low as 1 µU of G6PDH activity
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glucose-6-Phosphate Dehydrogenase (G6PDH: EC 1.1.1.49) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as liver, mammary gland, adipose tissue, and adrenal gland. BioVision’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces PicoProbe™ to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 µU of G6PDH activity.
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Test
Biovision supplies other types of Assays as 1.
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Conjugate
Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit
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Gene target
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Gene symbol
MIR1302-6, PIRC24, PIRC28, PIRC26, ADCP1, PIRC27, PIRC25
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Short name
™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-6
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Product images
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