Nucleostemin/GLN3 Antibody (CT)

• Catalog number
  6782-100
• Price
  Please ask
• Size
  100 μl

• Antibody Target
  Nuclerostemin
• Target Alternative Name
  GNL3; E2IG3; NS; Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47; Nucleolar GTP-binding protein 3; Nucleostemin
• Antibody Type
  Polyclonal
• Species Reactivities
  Human.
• Immunogen Sequence
  This Nucleostemin (GNL3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-487 amino acids from the C-terminal region of human Nucleostemin (GNL3).
• Accession
  Q9BVP2
• Gene Id
  26354
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Purification
  Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Application
  Western blot, IHC.
• Positive Control
  Western blot: HL60 cell line lysate, IHC: human hepatocarcinoma tissue.
• Application And Usages
  WB: 1:1000, IHC: 1:10 – 1:50.
• Description
  Nucleostemin, also designated Nucleolar GTP-binding protein 3, is a member of the MMR1/HSR1 GTP-binding protein family. It is expressed in the nucleoli of adult CNS stem cells, primitive bone marrow cells, and embryonic stem cells and in several cancer cell lines. Nucleostemin is often used as a stem cell marker. Overexpression or depletion of the protein can reduce cell proliferation in CNS stem cells. Nucleostemin shuttles between the nucleus and the nucleolus and may be important in maintaining the proliferative capacity of stem cells. Nucleostemin is important in the growth regulation of liver cancer, gastric cancer and several other cancer types. The gene encoding Nucleostemin is localized to chromosome 3p21.1.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Nucleostemin/GLN3  
• Short name
  Nucleostemin/GLN3 Antibody (CT)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Nucleostemin/GLN3 (antibody to-) (CT)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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