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Alternative_name
3,3',5,5'-Tetramethylbenzidine; TMBUS
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Description
3,3'5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mo⁻¹1 cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBUS, available from Biovision Inc., is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.
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CAS Number
54827-17-7
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Structure Available
No
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Salt Form
No
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Molecular Formula
C₁₆H₂₀N₂
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Molecular Weight
240.35
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Cell Permeable
No
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Handling
Protect from light
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Tag Line
A peroxidase substrate
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Storage Condition
+4°C
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Shipping Condition
RT
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Shelf Life
12 months
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MDL Number
MFCD00007748
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PubChem CID
41206
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SMILES
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
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InChi
InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
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InChi Key
UAIUNKRWKOVEESUHFFFAOYSA-N
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MSDS Available
Yes
