ACHE Antibody (CT) (Clone 684CT8.3.4)
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Catalog number
6707-100
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Price
Please ask
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Size
100 μl
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Antibody Target
ACHE
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Target Alternative Name
ACHE; Acetylcholinesterase
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Antibody Type
Monoclonal
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Clone
684CT8.3.4
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Species Reactivities
Human, mouse, rat
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Immunogen Sequence
This ACHE antibody is generated from mouse immunized with a KLH conjugated synthetic peptide between 587-611 amino acids from the C-terminal region of human ACHE.
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Accession
P22303
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Gene Id
ACHE
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
100 µl of antibody in PBS with 0.09% (W/V) sodium azide
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Purification
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Application
Western blot
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Positive Control
Western blot: Raji, Jurkat, COS7, NIH3T3 mouse and rat cerebellum lysates
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Application And Usages
Western blot: ~1:2000
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Description
Acetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions. Alternative mRNA splicing gives rise to three forms of AChE. It plays a role in neuronal apoptosis. The T form, also known as the asymmetric form, is soluble and is present in synapses. The H form is also known as the globular form and is present on the outer surfaces of cell membranes. The R form is not known to be a functional species. AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail. The catalytic subunits of AChE are oligomers composed of disulfide-linked homodimers. The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer’s disease. However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
ACHE
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Short name
ACHE Antibody (CT) (Clone 684CT8 3 4)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG1
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Alternative name
acetylcholinesterase (antibody to-) (CT) (clonality 684CT8.3.4)
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Alternative technique
antibodies
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Alternative to gene target
acetylcholinesterase, ACEE and ARACHE and N-ACHE and YT, ACHE and IDBG-32513 and ENSG00000087085 and 43, protein self-association, nuclei, Ache and IDBG-204838 and ENSMUSG00000023328 and 11423, ACHE and IDBG-640435 and ENSBTAG00000001139 and 540446
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Clone name
684CT8
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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