Anti-CD45 Rabbit Monoclonal Antibody

• Catalog number
  A1597-50
• Price
  Please ask
• Size
  50 µl

• Antibody Target
  CD45
• Target Alternative Name
  B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
• Antibody Type
  Monoclonal
• Clone
  RM291
• Species Reactivities
  Human
• Immunogen Sequence
  A peptide corresponding to the C-terminus of human CD45 (Receptor-type tyrosine-protein phosphatase C)
• Accession
  P08575
• Gene Id
  5788
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
• Purification
  Affinity purified
• Application
  WB, IHC
• Positive Control
  WB: Jurkat cell lysate, IHC: tonsil tissue section
• Application And Usages
  WB: 1:1000-1:2000, IHC: 1:100-1:200
• Description
  CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phospho-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  CD45   Monoclonal  
• Gene symbol
  PTPRCAP, PTPRC
• Short name
  Anti-CD45 Rabbit Monoclonal Antibody
• Technique
  Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Rabbit, Rabbits
• Isotype
  Rabbit IgG
• Alternative name
  Antibody toCD45 production species: rabbit monoclonal (antibody to-)
• Alternative technique
  antibodies, rabbit-anti

Gene info

• Identity
  HGNC:9667
• Gene
  PTPRCAP
• Long gene name
  protein tyrosine phosphatase receptor type C associated protein
• Synonyms
  • LPAP
  • CD45-AP
• Locus
  11q13.2
• Discovery year
  1996-04-22
• Entrez gene record
  5790
• RefSeq identity
  • NM_005608
• VEGA ID
  OTTHUMG00000150325

Gene info

• Identity
  HGNC:9666
• Gene
  PTPRC
• Long gene name
  protein tyrosine phosphatase receptor type C
• Synonyms gene
  • CD45
• Synonyms
  • LCA
  • T200
  • GP180
• GenBank acession
  • Y00062
• Locus
  1q31.3-q32.1
• Discovery year
  1986-01-01
• Entrez gene record
  5788
• Pubmed identfication
  • 2169617
• RefSeq identity
  • NM_002838
• Classification
  • Protein tyrosine phosphatases receptor type
  • Fibronectin type III domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000035702
• Locus Specific Databases
  • PTPRCbase: Mutation registry for CD45 deficiency
  • LRG_95

MeSH Data

• Name
  CD4 Lymphocyte Count
• Concept
  Scope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
• Tree numbers
  • E01.370.225.500.195.107.595.500.150
  • E01.370.225.625.107.595.500.150
  • E05.200.500.195.107.595.500.150
  • E05.200.625.107.595.500.150
  • E05.242.195.107.595.500.150
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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