FNDC3A polyclonal antibody
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Catalog number
BS90521-50ul
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Price
Please ask
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Size
50ul
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Alternative Name
fndc7a, Fibronectin type III domain containing protein 3a, FNDC3, HUGO, KIAA0970 antibody
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Immunogen
Recombinant protein within Zebrafish FNDC3A aa 100-310.
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Swiss Prot
F1R5M9 Zebrafish
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Reactivity
Zebrafish
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Applications
IF IHC
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Application_all
IF:1:50-1:200 IHC:1:50-1:200
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
Protein affinity purified.
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Storage Stability
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Specificity
FNDC3A polyclonal antibody detects endogenous levels of FNDC3A protein.
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Usage
Immunofluorescence staining of paraffin- embedded zebrafish tissue using anti-FNDC3A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1803-66 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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BiowMW
67 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
FNDC3A
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Short name
FNDC3A polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
fibronectin classification III domain containing 3A polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
fibronectin type III domain containing 3A, bA203I16.1 and bA203I16.5 and FNDC3 and HU this GO, FNDC3A and IDBG-32853 and ENSG00000102531 and 22862, poly(A) RNA binding, Plasma membranes, Fndc3a and IDBG-179575 and ENSMUSG00000033487 and 319448, FNDC3A and IDBG-629107 and ENSBTAG00000020681 and 508840
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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