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Available ordering format
Inquire
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Immunogen
KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
Monoclonal
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Purification
Protein A purified in PBS with 0.05 % sodium azide
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
chromatin immunoprecipiation (ChIP), western blot (WB)
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Connected products
AS16 3195 | anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade), rabbit antibodies AS16 3190-10 | anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) (10 µg), rabbit antibodies AS16 3187 | anti-H3K56me1 | Histone H3 (monomethyl Lys56), polyclonal antibodies AS16 3182 | anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies AS16 3183 | anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
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Recommended dilutions for use
1-2 µg/IP (ChIP), 1: 1000 (WB)
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Molecular weight expected аpparent
15 kDa
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Verified reactivity
human
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Possible reactivity
Solanum lycopersicum, Populus sp., Zea mays
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No reactivity
No confirmed exceptions from predicted reactivity known at the moment
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Supplementary information
To be added when available
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References
To be added when available, antibody released in February 2016.
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Scientific context
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
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Notes
Antibody has been purified using Protein A.Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
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Protein number
P68431
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TAIR number
Refer to NCBI
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
