-
Type
Conjugated Primary Antibody
-
Conjugated with
ALEXA FLUOR® 594
-
Host organism
Rabbit (Oryctolagus cuniculus)
-
Target Protein Peptide
T140
-
Specificity
This antibody reacts specifically with IFT140
-
Modification
No modification has been applied to this antibody
-
Modification site
None
-
Clonality
Polyclonal Antibody
-
Clone
Polyclonal Antibodies
-
Concentration
1ug per 1ul
-
Subcellular locations
N/A
-
Antigen Source
KLH conjugated synthetic peptide derived from human IFT140
-
Gene ID
N/A
-
Swiss Prot
N/A
-
Applications
IF(IHC-P)
-
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
-
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
-
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
-
Background information
IFT140 is a gene encodes one of the subunits of the intraflagellar transport (IFT) complex A. Intraflagellar transport is involved in the genesis, resorption and signaling of primary cilia. The primary cilium is a microtubule-based sensory organelle at the surface of most quiescent mammalian cells, that receives signals from its environment, such as the flow of fluid, light or odors, and transduces those signals to the nucleus. Loss of the corresponding protein in mouse results in renal cystic disease.
-
Purification method
Purified by Protein A.
-
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission
590nm/617nm
-
Synonyms
Gs114; Intraflagellar transport 140 homolog Chlamydomonas; WD and tetratricopeptide repeats protein 2; 140_HUMAN; WDTC2.
-
Also known as
IFT140 Polyclonal Antibody
-
Other name
Anti-IFT140 Polyclonal
-
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Properties
For facs or microscopy Alexa 1 conjugate.
-
Conjugation
Alexa Fluor, ALEXA FLUOR® 594
-
Conjugated
Alexa conjugate 1
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
Gene target
-
Gene symbol
IFT140
-
Short name
Anti-IFT140
-
Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Isotype
Immunoglobulin G (IgG)
-
Label
ALEXA FLUOR 594
-
Alternative name
IFT140 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
intraflagellar transport 140 homolog (Chlamydomonas), c305C8.4 and c380F5.1 and gs114 and MZSDS and SRTD9 and WDTC2, IFT140 and IDBG-9054 and ENSG00000187535 and 9742, protein binding, multiple, Ift140 and IDBG-150427 and ENSMUSG00000024169 and 106633, IFT140 and IDBG-634576 and ENSBTAG00000007245 and 100139697
-
