D-Tag Antibody

• Catalog number
  3989-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  DYKDDDDK Tag
• Target Alternative Name
  D-Tag, anti-D-Tag, D-Tag antibody,D-Tag antibody
• Antibody Type
  Polyclonal
• Species Reactivities
  All species
• Immunogen Sequence
  A synthetic D- tag peptide DYKDDDDK conjμgated to KLH.
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) affinity purified rabbit anti-D-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Positive Control
  MYC/D- tagged protein ladder (Lane 1)
• Application And Usages
  The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
• Description
  DYKDDDDK Tag antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Purpose Only. Not to be used in human subjects.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  D-Tag  
• Short name
  D-Tag Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for, Tags
• Isotype
  Rabbit IgG
• Label
  tag
• Alternative name
  D-detection labelled (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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