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Target antigen
HSF1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human Predicted to work with: mouse, rat
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Analyses
WB,IHC-P,IHC-F,ICC
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HSF1(380-397aa DKNELSDHLDAMDSNLDN), identical to the related rat and mouse sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the HSF1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The HSF1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
HSF1(HEAT-SHOCK TRANSCRIPTION FACTOR 1), also called HEAT-SHOCK FACTOR 1, is a protein that in humans is encoded by the HSF1 gene. The product of HSF1 gene is a heat-shock transcription factor. The International Radiation Hybrid Mapping Consortium maps the HSF1 gene to chromosome 8. HSF1 exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90. Upon stress, such as elevated temperature, HSF1 is released from the chaperone complex and trimerizes. HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements. HSF1's target genes include major inducible heat shock proteins such as Hsp72, and interestingly, noncoding RNA within Satellite III repeat regions. In a novel in vitro system human HSF1 can be activated by nonnative protein, heat, and geldanamycin. HSR1 is constitutively expressed in human and rodent cells and its homologs are functionally interchangeable. Furthermore, Hsf1-deficient mice had a longer free-running period than wildtype littermates.
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Related articles
1. Christians, E., Davis, A. A., Thomas, S. D., Benjamin, I. J. Maternal effect of Hsf1 on reproductive success. Nature 407: 693-694, 2000. 2. Schuetz, T. J. Personal Communication. Boston, Mass. 10/3/1991.
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Gene Name
HSF1
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Protein Name
Heat shock factor protein 1(HSF 1)
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Gene Full Name
heat shock transcription factor 1
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Synonyms
Heat shock factor 1 antibody|Heat shock factor protein 1 antibody|Heat shock transcription factor 1 antibody|HSF 1 antibody|hsf1 antibody|HSF1_HUMAN antibody|HSTF 1 antibody|HSTF1 antibody
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Uniprot ID
Q00613
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
