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Alternative name1
Anti-Triticum vulgare (WGA, Wheat germ) (FITC)
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Alternative name2
Triticum vulgare (WGA, Wheat germ) (FITC)
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Alternative name3
Triticum vulgare (WGA, Wheat germ) (FITC)
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Alternative name4
Triticum vulgare
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Alternative name5
Anti -Triticum vulgare (WGA, Wheat germ) (FITC)
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgG
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
N/A
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Specificity and cross reactivity
Recognizes Triticum vulgaris.
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Purification method
Purified by ammonium sulfate precipitation.
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Form Appearance
Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC). OD 495/280: 1.5
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Concentration
N/A
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Storage and shipping
Store at 4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, productone should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
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Tested for
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
