T4 RNA Ligase 2 (dsRNA Ligase)

• Catalog number
  M1219-100
• Price
  Please ask
• Size
  100 µl

• Description
  Catalyzes the ATP-dependent formation of a 3’→5’ phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. T4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3’→5’ phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 (M1218-100) which ligates single-stranded RNA, T4 RNA Ligase 2 joins nicks on double-stranded RNA and can also ligate the 3’OH of RNA to the 5’ phosphate of DNA in a double stranded structure.
• Product Highlights
  Catalyzes the ATP-dependent formation of a 3’→5’ phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining
• Storage Temp
  -20°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.

• Gene target
  RNA   Ligase   dsRNA   Ligase  
• Short name
  T4 RNA Ligase 2 (dsRNA Ligase)
• Technique
  RNAs are to store at -70 C
• Alternative name
  T4 RNA Ligase 2 (dsRNA Ligase)

MeSH Data

• Name
  Ligase Chain Reaction
• Concept
  Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
• Tree numbers
  • E05.393.620.311
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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