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Category
Primary Antibodies
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Long description
p21/WAF1, also known as cip1 and cyclin-dependent kinase interacting protein 1, is a 21kDa protein that belongs to the CDI family of cyclin-dependent kinase inhibitors. In humans p21/WAF1 is encoded by the CDKN1A gene, the transcription of which is regulated by P53, for example in the case of DNA damage. The p21/WAF1 protein exists within the nucleus as a complex with cyclin, cyclin-dependent kinase (cdk) and proliferating cell nuclear antigen (PCNA). It functions as a potent inhibitor of cyclin-dependent kinases and plays a critical role in regulating cell growth and the cellular response to DNA damage. Studies show that the amino-terminal domain of p21/WAF1 binds to cyclin/cdk complexes and inhibits their activity, preventing cell cycle progression at the G1/S phase. In addition, p21/WAF1 can also bind through its carboxy-terminal domain to PCNA, inhibiting the activity of PCNA and, as a result, DNA replication. Expression of p21/WAF1 is tightly regulated at the transcriptional level by the tumour suppressor protein p53. It is reported to be upregulated in response to a variety of cellular stress stimuli. Loss of p21 expression has been associated with poor prognosis in several carcinomas including gastric carcinoma, non-small cell lung carcinoma, and thyroid carcinoma.
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Antibody come from
WA1 is a mouse IgG1 monoclonal antibody derived from the fusion of SP2 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with human p21 protein.
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Other description
Each vial contains 100 µg 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Clone
WA1
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Antigen antibody binding interaction
Mouse anti p21/WAF1/ cip1/Cyclin-dependent kinase inhibitor 1 Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, non human primates
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
The antibody WA1 is directed against human p21/WAF1, but also recognizes this protein in non-human primates . Reports suggest that this antibody recognizes an epitope within the carboxy-terminal region of p21/WAF1.
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Research interest
CD Marker, Cell adhesion, Cancer
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Application
Flow cytometry, Immunocytochemistry, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting, ELISA
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Antibody s suited for
The WA1 antibody is suitable for the detection of p21/WAF1 by flow cytometry, immunocytochemistry, ELISA and western blotting. The antibody is also suitable for the detection of p21/WAF1 by immunohistochemistry of frozen and paraffin embedded tissues. Paraffin embedded tissues require pre-treatment prior to staining with the WA1 antibody. Antigen retrieval by heat treatment using sodium citrate buffer is suggested for this purpose. Optimal antibody dilutions for the different applications should be determined by titration.
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Storage
Store at 4oC, or in small aliquots at -20oC.
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Relevant references
1. Kolar Z, Murray PG, Scott K, Harrison A, Vojtĕsek B, Dusek J. (2000) Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer. Mol Pathol. 53:15-8. 2. Khanna SJ, Brown R, Whetton AD, Ball KL, Dive C. (2001) v-Abl protein-tyrosine kinase up-regulates p21WAF-1 in cell cycle arrested and proliferating myeloid cells. J Biol Chem. 276:11143-11150. 3. Kolar Z, Flavell JR, Ehrmann J Jr, Rihakova P, Macak J, Lowe D, Crocker J, Vojtesek B, Young LS, Murray PG. (2000) Apoptosis of malignant cells in Hodgkin's disease is related to expression of the cdk inhibitor p27KIP1. J Pathol. 190:604-12.
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Protein number
UniProt: P38936 (Human)
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
