Yeast Mitochondria Isolation Kit
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Catalog number
K259-50
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Price
Please ask
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Size
50 assays
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Description
Highly efficient kit to isolate intact yeast mitochondria
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Summary
Application • Isolation of highly pure intact mitochondria from yeast cells. • Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. • Western blot and ELISA
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Sample Type
Yeast cells culture.
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Features Benefits
• Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate intact mitochondria
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Mitochondria are the power house of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria is a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. BioVision’s Yeast Mitochondria Isolation kit will enable fast and easy purification of mitochondria from yeast cells, utilizing yeast cell wall lysis and homogenization. This Mitochondria Isolation Kit is tested for Pichia pastoris & Saccharomyces cerevisiae & can be used to isolate mitochondria from other yeast. Under fermentable media, the yield is ~150-200 µg of mitochondria & under non-fermentable media (e.g. Glycerol) ~200-250 µg of mitochondria from a culture of OD ~20.
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Gene target
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Short name
Yeast Mitochondria Isolation Kit
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Host
Yeast, Ascomycota
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Species
Yeast, Yeasts
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Alternative name
Yeast Mitochondria purification reagent
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
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Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
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