Two-step RT-PCR Kit

• Catalog number
  FTRP-2X25
• Price
  Please ask
• Size
  2X25 rxn

• Description
  Two-step RT-PCR Kit is used to synthesize the first-strand cDNA followed by amplification of the cDNA product using PCR.
• Similar product
• Storage
  NA
• Shelf life
  NA
• Contents
  M-MLV Reverse Transcriptase (200U/µl) ; 5×M-MLV Buffer ; 100 mM DTT ; dNTPs(10 mM each) ; RNasin (40U/µl) ; Oligo(dT)18 (20 pmol/µl) ; (dN)9 (20 pmol/µl) ; DEPC-treated water ; Taq DNA polymerase (5U/µl) ; 10×PCR Buffer
• Technical data
• Data sheet
  Contact us to request a Data sheet
• Ordering
  To order Two-step RT-PCR Kit, please use the Cat. Nr.FTRP-2X25 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for Two-step RT-PCR Kit
• Group
  PCR, polymerase chain reaction, RT-PCR mixes
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

• Gene target
  Two   Kit  
• Short name
  Two-step RT-PCR Kit
• Technique
  RT-PCR, step, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. steps
• Alternative name
  Two-step Reverse transcription PCR test kit reagent
• Alternative technique
  rtpcrkits, steps, kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Nucleic Acid Amplification Techniques
• Concept
  Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
• Tree numbers
  • E05.393.620
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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